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OneMinute®Plus
Western Blot Stripping Buffer
Longer
shelf life, and economy-size available |
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OneMinute®Plus
Western Blot Stripping Buffer has a little bit better stripping efficiency than
OneMinute® Western Blot Stripping Buffer, and much longer shelf life. It provides economy-size for those customers who prefer to spend less for a larger volume, but can not use it up in short
time.
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OneMinute®Plus
Western Blot Stripping Buffer immediately
makes your membrane ready for re-blotting in
ONE minute. It
spends only 30 seconds for stripping and 3x 10
seconds for washing.
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Highlights:
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- Longer
shelf life: ~18
months
- a
little bit higher efficiency than OneMinute®
Western Blot Stripping Buffer
- Faster, easier and more efficient: only
need 1 min
- No
significant loss of transferred
proteins
- Multiple times of re-probes: ~10
times
- Re-blocking is usually not necessary
- Perform at
RT, no offensive odor
- Work
for both nitrocellulose and PVDF
membranes
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Protocols:
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1.
Immerse membrane in OneMinute®Plus
Western Blot Stripping Buffer
for 30 sec.
2. Wash the blot with Washing Buffer
(TBST or PBST)
3 x 10 seconds*.
*The
blot is ready for re-probing. Re-blocking
membrane is usually not necessary. |
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Fig
10. OneMinute®Plus is a bit better than OneMinute® stripping buffer, OneMinute®Advance has the highest stripping efficiency.
293 cell lysate was probed with high-affinity actin antibody (left panel). Blots were then stripped with OneMinute®
Stripping Buffer, or OneMinute®Plus Stripping Buffer, or PBST control (-), or
OneMinute®Advance Stripping Buffer. The blots were then re-probed with 2nd antibody to test the presence of actin antibody(Right panel). Please
click the figure for details. |
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Related
Products and Links
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Ordering
Information |
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