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OneMinute®Advance
Western Blot Stripping Buffer
2nd
generation especially for high-affinity antibodies
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OneMinute®Advance Western Blot Stripping Buffer has
extraordinary efficiency to strip off high-affinity
antibodies without loss of transferred proteins, which
ensures multiple times re-probing.
Re-blocking
is NOT necessary and the blot is ready for re-probing in 6
min (5 min pretreatment and 1 min stripping). It
represents the world-best Western Blot stripping buffer.
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Highlights:
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- Especially
for high-affinity antibodies
- Fast
and easy
- No
significant loss of transferred
proteins
- Multiple times of re-probes: ~10
times
- Re-blocking is usually not necessary
- Perform at RT, no offensive odor
- Work
for both nitrocellulose and PVDF
membranes
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Protocols:
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1. Pretreatment of blot with Stripping Enhancing
buffer for 5min
2.
Immerse membrane in OneMinute®
Western Blot Stripping Buffer for 30 sec.
3. Wash the blot with Washing Buffer (TBST or PBST)
3 x 10 seconds*.
*The
blot is ready for re-probing. Re-blocking
membrane is usually not necessary. |
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8. OneMinute®Advance is more
efficient than Pierce’s Restore and RestorePlus
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| 293 cell lysate was probed with high-affinity
mouse-anti-human actin antibody (left panel). Blots were then stripped either with
OneMinute®Advance Stripping Buffer (5 min pretreatment + 1min stripping) or Pierce’s Restore and Restore Plus (15min stripping). The blots were then
re-probed with mouse-anti-human RIPK1 antibody (Right panel).
Please click the figure for details. |
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Related
Products and Links
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Ordering
Information |
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| Catalog# |
Description |
Size |
Price |
Document |
| GM6031 |
OneMinute®Advance Western Blot Stripping Buffer
(Sufficient
for stripping 25 blots of 8cm x10cm)
Components: OneMinute® WB Stripping
Enhancing Buffer (Cat# GM6021)
OneMinute® Western Blot Stripping Buffer
(Cat# GM6001) |
25
Tests |
$169.00 |
Protocol
MSDS
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