Our innovative technology of site-directed mutagenesis enables us to perform up to 60bp insertion/substitution in a single reaction with unparalleled accuracy. Mutagenesis services is following:
  • insert tags such as Flag, Myc, HA, 6His, V5......

  • delete tags such as Flag, Myc, HA, 6His, V5......

  • switch tags such as Flag, Myc, HA, 6His, V5......

  • insert/delete/switch any tag (fragment) anywhere of a gene

  • insertion of any sequence up to 60bp in a single reaction at any desire site

  • deletion of any sequence up to 60bp in a single reaction at any desire site

  • substitution of any sequence up to 60bp in a single reaction at any desire site

  • >60bp insertion/deletion/substitution needs multiple reactions

  • re-construct MCS sites (add/delete/replace up to 10 RE site)

  • insert/delete/switch tags such as Flag, Myc, HA, 6His, V5

  • adjust reading frame

We will perform

  1. Design and synthesis of mutagenic primers

  2. Perform mutagenesis reaction, transformation, clones culturing

  3. DNA preparation, sequence multiple clones to confirm the mutation*

  4. Shipping 1-5 microgram of purified plasmid, DNA sequencing data alignment result and sequencing primer 

    Average Turnaround time: 8-14 business days

    *A 500 bp region containing the mutation site will be sequenced to confirm the desired mutation. If customer requests a full-length DNA sequencing, we will charge $70 per 1kb for primer synthesis and sequencing.

 

We are committed to 100% customer satisfaction guarantee.  
If you receive a plasmid from GM Biosciences with error(s), we will  correct the error(s) with no additional charge. Please click here for details.  

Customer Provides

  1. One microgram of plasmid

  2. information of vector and target gene

Data Security

From start to finish, GM Biosciences takes the security of your data very seriously. We guarantee the complete confidentiality of the sequence data and other information. All materials and information received from customers will be held in the strictest confidence.
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