Researchers usually suffer either significant loss of transferred proteins or incomplete removal of the antibodies when stripping western blot membranes. OneMinute® and OneMinute®Advance Western Blot Stripping Buffer were developed to effectively strip off high-affinity antibodies without loss of transferred proteins on the membrane

        OneMinute® Western Blot Stripping Buffer
  • Blot is ready for re-probing in ONE minute
  • Gentle to transferred protein ensuring ~10 times of re-probing
  • Re-blocking generally unnecessary
        OneMinute®Advance Western Blot Stripping Buffer
  • The 2nd generation formulation especially for stripping off high-affinity antibodies
  • Gentle to transferred proteins ensuring ~10 times of re-probing
  • Re-blocking generally unnecessary
        OneMinute® WB Stripping Enhancing Buffer
  • Dramatically increase the stripping efficiency of Western Blot stripping reagents.
Western Blot Stripping Buffer Selection Guide
                     
----Compare OneMinute /OneMinuteAdvance and Pierce's Restore /RestorePlus
  OneMinute OneMinute Advance Pierce's Restore Pierce's RestorePlus
Stripping efficiency +++ ++++ + ++
Cost per test $4.36
(25 tests/$109)
$6.76
(25 tests/$169)
$4.48
(25 tests/$112)
$4.48
(25 tests/$112)
Total time before re-probing (Stripping, Re-blocking, Washing) 1min 6min 120min 120min
Stripping specificity +++ ++++ + ++
Multiple re-probing 10 times 10 times 2 times 2 times
Re-blocking No No Yes Yes
Harsh to transferred proteins No No Yes Yes
 

 

 

 

 

Primary benefits over other competing buffers: 

OneMinute® Western Blot Stripping Buffer: 
economy, high efficiency, time-saving (1min stripping, re-blocking unnecessary), multiple times re-probing. 
OneMinute®Advance Western Blot Stripping Buffer: 
highest efficiency, time-saving (6min stripping, re-blocking unnecessary), multiple times re-probing
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