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Researchers usually suffer either significant loss of transferred proteins or incomplete removal of the antibodies when stripping western blot membranes. OneMinute®, OneMinute®Plus and OneMinute®Advance Western Blot Stripping Buffer were developed to effectively strip off high-affinity antibodies without loss of transferred proteins on the membrane
        OneMinute® Western Blot Stripping Buffer
  • Blot is ready for re-probing in ONE minute
  • Gentle to transferred protein ensuring ~10 times of re-probing
  • Re-blocking generally unnecessary
        OneMinute®Plus Western Blot Stripping Buffer
  • Blot is ready for re-probing in ONE minute
  • Gentle to transferred protein ensuring ~10 times of re-probing
  • Re-blocking generally unnecessary
  • Longer shelf life
  • Large/Bulk size available
        OneMinute®Advance Western Blot Stripping Buffer
  • The 2nd generation formulation especially for stripping off high-affinity antibodies
  • Gentle to transferred proteins ensuring ~10 times of re-probing
  • Re-blocking generally unnecessary
        OneMinute® WB Stripping Enhancing Buffer
  • Dramatically increase the stripping efficiency of Western Blot stripping reagents.
Selected Citations for OneMinute® Products
1. Nat Chem Biol. 2010 Jul;6(7):541-8. Structural basis of G protein-coupled receptor-G protein interactions. PMID: 20512139

2. Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15951-6. AMPA receptors are exocytosed in stimulated spines and adjacent dendrites in a Ras-ERK-dependent manner during long-term potentiation. PMID: 20733080

3. Int Immunol. 2011 Oct 24. Human eosinophils express RAGE, produce RAGE ligands, exhibit PKC-delta phosphorylation and enhanced viability in response to the RAGE ligand, S100B. PMID: 22025532

4. PLoS Genet. 2011 May;7(5):e1002083. Meiotic recombination intermediates are resolved with minimal crossover formation during return-to-growth, an analogue of the mitotic cell cycle. PMID: 21637791

5. Mol Microbiol. 2011 Sep;81(5):1190-204. doi: 10.1111/j.1365-2958.2011.07742.x. Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. PMID: 21696463

 
Western Blot Stripping Buffer Selection Guide
                      ----Compare OneMinute /OneMinuteAdvance and Pierce's Restore /RestorePlus
  OneMinute® OneMinute® Plus OneMinute® Advance Pierce's Restore Pierce's RestorePlus
Stripping efficiency +++ +++ ++++ + ++
Cost per test $4.36
(25 tests/$109)		 $4.20
(100 tests/$420)		 $6.76
(25 tests/$169)		 $4.64
(25 tests/$116)		 $4.64
(25 tests/$116)
Total time before re-probing (Stripping, Re-blocking, Washing) 1min 1min 6min 120min 120min
Stripping specificity +++ +++ ++++ + ++
Multiple re-probing 10 times 10 times 10 times 2 times 2 times
Re-blocking No No No Yes Yes
Shelf Life ~6 months ~18 months ~6 months N/A N/A
Harsh to transferred proteins No No No Yes Yes
 

 

 

 

 
Primary benefits over other competing buffers: 

OneMinute® and OneMinute®Plus Western Blot Stripping Buffer: 
economy, high efficiency, time-saving (1min stripping, re-blocking unnecessary), multiple times re-probing. 
OneMinute®Advance Western Blot Stripping Buffer: 
highest efficiency, time-saving (6min stripping, re-blocking unnecessary), multiple times re-probing
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