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GM Biosciences Inc. is a research based biotech company dedicated to developing innovative techniques and products for life science researches.  

　

We will strive to provide products that are of the highest quality.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
401 Rosemont Avenue
3rd Fl. Rosenstock Hall
Frederick, MD 21701
USA
　

　

OneMinute Western Blot Stripping Buffer

Suffering significant loss of transferred proteins from stripping your blots? Getting tired of tedious procedure of stripping? OneMinute Western Blot Stripping Buffer is your best choice.

Highlights:
  • Faster and easier: < 1 min
  • Multiple times of re-probes (~10 times)
  • Re-blocking is usually not necessary
  • Perform at room temperature
Protocols:
1. Immerse membrane in OneMinute Western Blot Stripping Buffer for 30sec.
2. Wash the blot with Washing Buffer (TBST or PBST) 3 x 10 seconds*.
*The blot is ready for re-probing. (Note: Reblocking membrane is usually not necessary.)

OneMinute Stripping
WB membrane
 <1min
Stripping
(30sec, RT)
Wahsing
(3x10sec)


　


　
Re-blot
to primary antibody

Traditional Stripping
WB membrane

2-3h
Stripping
(30 min, 50℃)
Washing
(3x15min)
Re-blocking
(60 min)
Washing
(3x15min)
Re-blot
to primary antibody
 

 

            Mutagenesis Kit
GM Biosciences is developing a novel kit of Site-Directed Mutagenesis. Powered by innovative technology, the kit is capable of: 
  • site-directed mutagenesis 
  • insert or switch a tag (Flag, Myc, HA, 6His, V5 ... ...) into a gene or a vector
  • re-construct MCS of a vector
  • insert or switch a signal peptide or protein domain anywhere in a gene
              
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