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OneMinute® Advance Western Blot Stripping Buffer

Our 2nd generation formulation, especially for high-affinity antibodies. It has extraordinary efficiency to strip off antibodies without loss of transferred proteins, ensuring multiple re-probes.

Highlights

  • World-Best Stripping Efficiency: Especially for high-affinity antibodies.
  • Fast and easy: Blot is ready for re-probing in 6 min (5 min pretreatment + 1 min stripping).
  • No significant loss of transferred proteins.
  • Multiple times of re-probes: ~10 times.
  • Re-blocking is usually not necessary.
  • Perform at RT, no offensive odor.
  • Work for both nitrocellulose and PVDF membranes.

Protocols

1. Pretreatment of blot with Stripping Enhancing buffer for 5min.
2. Immerse membrane in OneMinute® Western Blot Stripping Buffer for 30 sec.
3. Wash the blot with Washing Buffer (TBST or PBST) 3 x 10 seconds*.
*The blot is ready for re-probing. Re-blocking membrane is usually not necessary.

Experimental Data

Figure 8 Data

Fig 8. OneMinute® Advance is more efficient than Pierce’s Restore and RestorePlus. 293 cell lysate was probed with high-affinity mouse-anti-human actin antibody (left panel). Blots were then stripped and re-probed with mouse-anti-human RIPK1 antibody (Right panel). Please click the figure for details.