All Products / WB Stripping Buffer / OneMinute®

OneMinute® Western Blot Stripping Buffer

In ONE minute, your membrane is ready for re-probing. This revolutionary stripping buffer is designed to specifically strip off antibodies without significant loss of other non-antibody proteins.

Highlights

  • Faster, easier and more efficient: only need 1 min (Fig 2, 3)
  • No significant loss of transferred proteins (Fig 1, 5)
  • Multiple times of re-probes: ~10 times (Fig 6)
  • Re-blocking is usually not necessary
  • Perform at RT, no offensive odor
  • Work for both nitrocellulose and PVDF membranes (Fig 4)

Protocols

1. Immerse membrane in OneMinute® Western Blot Stripping Buffer for 30 sec.
2. Wash the blot with Washing Buffer (TBST or PBST) 3 x 10 seconds*.
*The blot is ready for re-probing. Re-blocking membrane is usually not necessary.

Experimental Data

Harsh stripping buffer (2%SDS, 100mM 2-ME, 50mM Tris, pH6.8, perform at 50C for 15min) always leads to significant loss of transferred proteins, which results in dramatically signal loss in subsequent re-blotting. When using mild stripping buffer such as Restore, researchers always suffer incomplete removal of antibodies, especially primary antibodies. OneMinute® Western Blot Stripping Buffer represents the only buffer available to efficiently remove antibodies (Fig 2, 3, 4), whereas keeping transferred proteins on the membrane in maximum (Fig 1, 5, 6).

Fig 2 shows that stripping with OneMinute® for half minute completely removed both secondary antibodies (panel B) and primary antibodies to protein 1 (panel C). In contrast, stripping with Restore for 15 minutes failed to strip off primary antibodies (panel C). This figure demonstrates the superb stripping efficiency of OneMinute®. The ability to strip off high-affinity antibodies by OneMinute® is much better than Restore (Fig 3).

Figure 1 Data

Fig 1. OneMinute® prevents the loss of transferred proteins comparing to traditional stripping buffer. Please click the figure for details.

Figure 2 Data

Fig 2. OneMinute® is much more efficient than Restore. Please click the figure for details.

Figure 3 Data

Fig 3. OneMinute® is much more efficient than Restore. Please click the figure for details.

Figure 4 Data

Fig 4. OneMinute® woks not only on nitrocellulose membrane but also on PVDF membrane. Please click the figure for details.

Figure 5 Data

Fig 5. Minimal loss of transferred proteins was achieved by OneMinute®. Please click the figure for details.

Figure 6 Data

Fig 6. Multiple times stripping (10x) by OneMinute® retained enough transferred proteins on membrane. Please click the figure for details.

Figure 7 Data

Fig 7. Detect phosphorylated and non-phosphorylated forms of the same protein by stripping with OneMinute®. Please click the figure for details.