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PathwayScan™ Multi-Pathway Reporter Kit

Making new discoveries has never been easier. This kit provides a comprehensive collection of proprietary luciferase reporters for 46 transcription factors, allowing you to scan 46 signaling pathways in a single experiment.

Highlights

  • Looking for novel signaling pathways in which a gene is involved
  • Identifying signaling pathways influenced by a mutant gene, microRNA, siRNA, or shRNA
  • Studying crosstalk of signal pathways
  • Identifying signaling pathways influenced by various stimuli (cytokines, chemokines, chemicals, hormones, etc.)
  • Signaling pathway profiling in a variety of scenarios
  • Screening signaling pathways influenced by home-made cells (transient or stable, overexpressed or knock-down, knock-out or knock-in)
  • Identifying the phenotype of transgenic, knock-out or knock-in mice
  • Drug screening

Benefits

  • Detects both activation and inhibition of transcription factors—a huge benefit rarely achieved by other methods.
  • Simultaneously screens 46 different signaling pathways relevant to cancer, immunology, stem cells, etc.
  • A single experiment can lead to many new discoveries (see data below).

Experimental Data

Figure 1 Data

Fig 1. EGF stimulated HEK 293 cells were analyzed by PathwayScan™ Multi-Pathway Reporter Kit. Cytokine EGF is known to activate transcription factors such as Erk, AP-1, STAT3, EGR-1 and GATA. In one single experiment, the activations of Erk (66-fold), AP-1 (4-fold), STAT3 (3.5-fold), EGR-1 (9.7-fold) and GATA (3.3-fold) were confirmed. In addition, 4 transcription factors were found for the first time to be activated or inhibited by EGF (labeled as “New discovery”).

Figure 2 Data

Fig 2. IKK2-CA overexpressed HEK 293 cells were analyzed by PathwayScan™ Multi-Pathway Reporter Kit. Protein kinases are involved in most signal transduction pathways. It is known that IKK2 activates NFκB. A single test on IKK2 not only confirmed its activation on NFκB (25-fold) and Erk (2.8-fold), but also revealed for the first time that 3 transcription factors were remarkably activated and 2 others were strongly inhibited (labeled as “New discovery”).

Figure 3 Data

Fig 3. Optineurin overexpressed HEK 293 cells were analyzed by PathwayScan™ Multi-Pathway Reporter Kit. Pathway-specific stimulus is routinely employed if a gene/treatment has an inhibitory effect. In contrast, PathwayScan™ is so sensitive that inhibition is detectable even without TNF stimulation. As shown, NFκB was successfully inhibited 9-fold by OPTN (green arrow). Moreover, 4 other transcription factors were found to be dramatically inhibited or activated (labeled as “New discovery”).