Fig 3. TagMaster^® competent cells achieve significantly higher mutagenesis efficiency than DH5α competent cells.

Mutagenesis reactions (2bp substitution and 14bp deletion) were performed by using 1U TagMaster Enzymes and 20ng of 8.1kb template plasmids under the conditions of 95°C for 5min, followed by 18 cycles of (95°C 20s, 60°C 10s, 68°C 4min). Without any treatment, the products were directly transformed into TagMaster competent cells or DH5α competent cells. 50-60 clones from each plate were picked, cultured, miniprepped and sequenced to verify the desired mutations, and the mutagenesis rates were thus determined. The results showed that TagMaster competent cells achieved a significantly higher mutagenesis rate than DH5α competent cells.