|
|
|
|
MicroRNAs
(miRNAs) are small, non-coding single stranded RNAs, and 21-23 nt in length. They have been implicated in many biological processes and disease development. miRNAs have been widely used in regulating gene expression by employing miRNA expression plasmids since its discovery. After transfection into mammalian cells, the miRNA expression plasmid is processed into mature
microRNAs in multiple steps:
 |
Drosha digests the
Pri-miRNA to release a Pre-miRNA which is
approximately 70nt with 25-30bp stem and a
relatively small loop.
Dicer cleave the
Pre-miRNA to form ~22 nt mature miRNA. |
|
|
|
The traditional way to construct a miRNA expression plasmid is to clone the
pre-miRNA sequences into a miRNA expression vector, which needs intensive labor and expert skill of molecular biology. It is also true for constructing a shRNA expression
plasmid.
|
|
| Powered
by our unique technology, we are able to switch
pre-miRNA/shRNA sequences in your miRNA/shRNA expression
plasmid. You will have a different miRNA expression plasmid to knock down
a different gene expression with an affordable price.
|
|
|
|
Basic
services
|
|
|
-- Switch Pre-miRNA sequences in your miRNA expression plasmid
-- Switch shRNA sequences in your shRNA expression plasmid
-- Insert pre-miRNA sequence into your miRNA expression vector
-- Insert shRNA sequenc into your shRNA expression vector
-- Plasmid size <= 8 kb (please contact
us if the plasmid size >8kb)
-- Turnaround time: 8-14 business days (from the date of receiving plasmid)
-- 100% satisfaction guarantee
-- 35% Introductory discount till Dec. 31, 2011
|
|
| |
Catalog
#
|
Description
|
Price
|
Discount
Price**
|
| . |
GM3001 |
Switch
microRNA/shRNA (<=70bp) |
$439 |
$285 |
| . |
GM3002 |
Switch
microRNA/shRNA (>70bp) |
Please
contact
us |
| . |
For
specific requests that vary from the routine, please contact
us . All prices subject to change. |
|
|
|
|
**Discount
Price is good through
December
31st, 2011 |
|
