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Cost Comparison

Many researchers generate their mutants by using commercial mutagenesis kit. However, long sequence(>30 bp) insertion/ deletion/ substitution can not be achieved by using commercial kit.

it is also very common to fail mutagenesis reaction for a special plasmid containing GC-rich region, tight secondary structure region, etc. The success of mutagenesis critically depends on the skills of researcher.

Moreover, Commercial kit is very costly. We take a simple point mutation for example:
  　  1 mutation  5 mutations
Commercial
Kit		 Mutagenesis kit $25 $25 x 5 = $125
Other Costs
 (primer, comeptent cell, miniprep kit, sequencing)		 $150 $150 x 5 = $750
1-2 day labor $300 $300
Total $475 $1175
Price of
other service company		 Total $250~$400 $1250~$2000
Price of
GM Biosciences		 Total $197* $936**
                  *Price is after 10% introductory discount    
                 **Price is after 10% introductory discount and additional 5% reaction discount
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